Any difference between "hydroxy naphtol blue", and "hydroxy naphtol blue , disodium salt" for LAMP?

More Alessandro Crimi's questions See All

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

SAR X-Band constellation from Capella Space?

Dear all, I'd like to open here a sort of forum for understanding how the geodesists community is moving in view of the X-band SAR satellite constellation. The new constellation will offer new...

24 January 2021 6,248 0 View

Colab - how to extract NDVI time series from Sentinel-2?

Hi all, I have a collection of Sentinel-2 images and several points within it. In Google Colab, I would like to extract NDVI time series over these points through the whole collection and display...

12 January 2021 4,044 3 View

The use of Orange, a high level tookit for Machine Learning, data mining and data science for scientific research or just teaching?

Related to data mining/data science, I recently discovered Orange (https://orange.biolab.si/) a high level very powerful free toolkit. Using it is very simple and you can test different models in...

09 December 2020 8,832 4 View

How to defend against the reviewer's "not enough trials" objection (non-parametric observed power issues)?

Hi everyone, we need some help with a request from a reviewer. Here's the issue: In our study we investigated the impairment in the ability to recognize facial emotions when a facemask is present...

05 November 2020 1,238 18 View

Estimate Enterprise Value using specific accounting (items/lines) variables in regressions?

Need help in finding the best papers related to the above question. Thank you.

30 August 2020 8,430 12 View

Effects of grammatical phrasing (2nd person singular vs. 1st person plural) on compliance to suggestions/prohibitions/prescriptions, etc?

Hi guys! I have a question on a framing effect-like issue. Well, everyone of us has the immediate feeling that there's a huge difference between saying - for instance - "you should respect the...

20 August 2020 7,859 6 View

How can particle fillers increase the elongation at break of polymers?

I am investigating some biocomposites formed by PBAT and tiny particle fillers derived from natural wastes. I have noticed that with a loading of 10%wt. of these fillers, the elongation at break...

28 July 2020 9,367 1 View

How to change features size without padding in CNN?

Hi, I'm using a 2d CNN. My input size is a square image of 20x20 pixels and my desired output has size 400x3. The most common thing I see is to introduce padding over the convolutional layers...

20 April 2020 2,947 4 View

Homemade Virus Zero technology to contrast Coronavirus diffusion: is it possible to homemade realize?

I would like to create in-house air purifiers that use Samsung's Virus Zero technology. In this way, the air in hospital environments could be sanitized easily, quickly and safely. In addition,...

21 March 2020 5,886 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View