Follow NREL protocol

For Determination of Structural Carbohydrates and Lignin in Biomass: NREL/TP-510-42618.

http://www.nrel.gov/biomass/pdfs/42618.pdf

Cellulose and solution just do not go together; either you have some very specific  highly chaotropic solvent (like MMNO) or cellulose just stays cristalline. To quantify it you need to hydrolyse it. Same for lignin. The NREL procedure is a variant of a Saeman hydrolysis, that is it degrades cellulose and lignin using concentrated sulfuric acid and what you quantify is glucose and lignin monomers.

I do not get 100 % mass balance closure  for pretreated biomass composition by NREL LAP protocols.  Every time it comes around 85-90% of total cellulose, hemicellulose, lignin and ash. what else remaining 10%. Please suggest someone.

Cellulose will be desolved in cupriaetheylinediamine (  CED )

I do not get 100 % mass balance closure  for pretreated biomass composition by NREL LAP protocols.  Every time it comes around 85-90% of total cellulose, hemicellulose, lignin and ash. what else remaining 10%. Please suggest someone.

I recommend you to read the articles:

Justin B. Sluiter, Raymond O. Ruiz, Christopher J. Scarlata, Amie D. Sluiter and David W. Templeton, Compositional Analysis of Lignocellulosic Feedstocks. 1. Review and Description of Methods, http://pubs.acs.org/doi/abs/10.1021/jf1008023

David W. Templeton, Christopher J. Scarlata, Justin B. Sluiter and Edward J. Wolfrum, Compositional Analysis of Lignocellulosic Feedstocks. 2. Method Uncertainties, http://pubs.acs.org/doi/abs/10.1021/jf100807b

Best regards,

Hi

you can follow the acetyl bromide method

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0110000

this is a good and simple method.