Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

Naming the "UK coronavirus variant" rises to the occasion?

In the context of the COVID-19 infodemic, the global profusion of monikers and hashtags for "UK coronavirus variant" have found their way into daily communication and contributed to a backlash...

01 January 2021 7,850 2 View

Number of Estimating Equations Required to Test Hypotheses?

After literature review, current draft as below: Variables: Independent = B (represented by I, M, O, S, C), Moderating = W Dependent = F Control = CV Hypotheses: H1a. I has significant...

18 September 2020 7,055 2 View

Why is my protein a smear when I detect with CoIP lysis buffer plus LDS?

Hi I am trying to express my protein R in tobacco leaves and do a CoIP, but I can't detect the protein R in CoIP input. I took the same tissue and added 8M Urea plus LDS protein buffer, protein R...

31 August 2020 9,534 10 View

What's the main difference between #Tris-glycine #SDS-PAGE and #Bis-Tris #SDS-PAGE. How pH and ion strenght affect SDS-PAGE?

sometimes, there is some smear on the SDS-PAGE, this can be eliminated with a different running buffer or a different PAGE, or a different way of sample loading buffer. how these affect the SDS-PAGE?

09 August 2020 6,149 3 View

Is there a gentle method to reduce cuprous oxide to copper while maintaining the original morphology?

Hello, Recently, I have synthesized some cuprous oxide nanomaterials and want to reduce them to metal while maintaining the morphology. Is there a way to do this?

04 August 2020 4,850 3 View

Is there a function coded by matlab to reproject the geographic raster data?

Is there a function coded by matlab to reproject the geographic raster data?especially for the case converting the Ease-Grid 2.0 to the geographic coordinate.

17 June 2020 7,875 2 View

Why the contaminated colony grow around the plate?

Doing the antibacterial test, the unknown colony grows around the plate. E. coli and antibacterial regent were mixed with LB, PBS and EDTA. Not likely the contamination was from the LB, PBS or...

18 May 2020 1,311 3 View

How to do a two-way ONOVA in SPSS?

I don't have advanced knowledge of SPSS and tutorials aren't making much sense to me, if anyone is able to explain SPSS two-way ONOVA step-by-step in the simplest form I would be grateful. My...

30 March 2020 8,769 2 View

Statistical tests for dummies - one way or two way onova?

I'm currently working on my under-grad dissertation project, researching whether Raffinose impacts gut barrier function. I have used a control measure and a high, medium and low dose of Raffinose...

24 March 2020 4,220 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View