Any advice on how to increase metastasis in zebrafish?

06 April 2021 1 6K Report

I am injecting pancreatic cancer cell lines into zebrafish that have been published to have a high metastatic rate; however, I am getting low levels of metastasis and often no metastasis. I am stimulating with HGF, waiting for 2-3dpi, keeping injected fish at 33'. Has anyone got any advice/thoughts on this? Thanks in advance!

Nima Kouchak

How can I contact you charly?

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