For months I have been trying to perform a chip in my lentivirus infected cells.
I have had huge problems regarding the sonication. It is never the same and I have too much genomic DNA. In particular, after 30 second cycles (30 secs. on, 30 secs. off) my smear is still spread too much. I am using a kit made by a company from Belgium, and working with brain and kidney cancer cells. To be sure that the sonicator was not the problem I sheared some genomic DNA, which gave me a very good results. Does anybody else have the same problem, or any advice?
Hongjuan Cui
It's nothing to do with lentivirus infection, my students always use diffent conditions in even in different cell type. there is no short cut. Just try! Good luck!
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