beside ELISA, anyone knows any other instruments to determine antibody concentration and a linear standard curve? I want to generate a standard with R value of close 0.99.
I have formulated antibody in semi solid dosage form and want to characterise its stability profile. Can someone help me how to quantify the amount of antibody in the formulation? how to get the...
06 July 2016 4,310 5 View
Can someone please suggest me an effective way to test the stability of the semi solid formulation of antibody. I have been using ELISA to test the formulation at different temperature storage but...
05 June 2016 3,339 2 View
I have been performing antibody stability test at different solvents and temperature. My solvents were glycerol, DMSO, Tween 20, PEG. I stored the antibody solution with these solvents in room...
04 May 2016 9,849 7 View
I am using Indirect ELISA to determine the concentration of my antibody and generate standard curve, however this method cant pick up concentration differences precisely. is there any other...
04 May 2016 951 18 View
I have prepared pluronic gel formulation and evaluated its biocompatbility against Human Fibroblasts. Given this polmer is FDA approved for drug formulation, i found it was toxic towards cells....
01 January 1970 6,989 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View