JUN and ABY dye are not available in Biorad machine. Can any one tell me which dye (present in biorad machine) wavelength is similar with JUN n ABY ??

For covid19 diagnosis TaqPath kit is having ABY n JUN dye... But in our lab Biorad Cx96 machine is available where ABY n JUN dye are not available. How can we use the Biorad machine for covid19...

22 July 2020 1,978 2 View

Is it possible to find the sensitivity of RMSD and RMSF in MD simulation through gromacs ?

Is it possible to find the regions of the protein structures most susceptible to temperature impact in terms of RMSD and RMSF , during MD simulation ?

06 May 2018 8,855 3 View

Can anyone suggest me where and how to apply for post-doc as my work is on utilization of Water Hyacinth

26 July 2017 3,040 3 View

What should be the ideal settings for HSP length cut-off and HSP-Hit coverage for running Blast in Blast2Go?

I have to analyze PacBio transcriptome data with four bin sizes: 1-2 Kb, 2-3 Kb, 3-6 Kb and 5-10 Kb. I can understand that the HSP length cut-off and HSP-Hit coverage settings must be customized...

26 July 2017 1,346 1 View

Can anyone identify the two plant species

The plants were collected from Barak Valley Assam

24 May 2017 9,971 6 View

By which means can the porosity be determined in a vegetal fibre ?

We develop a new mechanical characterization test for vegetal fibres. We need to know the apparent density of them. We can know easily the real density by pycnometry. We want to determine the...

12 April 2016 7,592 8 View

What are the advantages and the drawbacks of AFM and nanoindentation ?

I need to measure the mechanical properties of a composite material on its surface (so there are several material phases). Which method is better between AFM and nanoindentation ?

03 September 2013 2,030 9 View

Sample making for ELISA

At first I treated interferon beta protein to the tumor bearing mouse then I collected the tumor and blood from the mouse. I already made the tumor sample with PBS and collected serum from blood....

01 January 1970 7,883 4 View

ELISA sample preparation

I want to check interferon beta protein concentration in mouse tissue sample like heart, liver, lung and kidney. Can anyone please help how can I make the sample from the tissue for ELISA?

01 January 1970 1,848 3 View

ELISA standard curve

when we calculate the concentration of unknown samples based on standard curve, which range of data can be used? Lowest and highest range?

01 January 1970 2,748 5 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View

Solar energy production forecasting ?

Hello everyone! I am interested in the topic of solar energy production forecasting for a ground-mounted PV system of more than 100MW capacity. Can anyone please suggest what are the main data...

08 February 2021 6,834 5 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View