Here is a dilemma: there is a line of null mice (global knockout), which are null by genotyping and by mRNA analysis (qPCR). They were generated by replacement of an entire protein-coding region with a Neo cassette using homologous recombination. However, commercial antibodies raised to the N-terminus of this protein still detect it (on western blot). The signal is comparable to that in wild-type mice, with regards to the protein size (kDa) and band intensity. Thoughts?

More Yulia Dzhashiashvili's questions See All
Similar questions and discussions