Hello,
I'm looking for an antibody against HNF4A that work for pancreas (that label HNF4A in the beta cells), I tried one from sigma and 3 from abcam, it works well in the liver but no signal in the pancreas.
If somebody can give me a antibody reference and/or some tips to make it work.
Thanks,
Tom
I use IF and tissue, on extracted pancreatic's cells it works but not on tissue's slide
fixed in PFA, concerning the primary, I let them overnight at 4°C and before that I boil the slide in acide citric, sodium citrate buffer.
And yes I permeabilize with triton
Do you think that I should let the primary just 2 hours ?
Thanks,
tanks for your tips, just few question, you permeabilized with port K before the fixation with PFA 4 % or after ?
And what il the concentration of the proteinase K ?
Thanks again
Tom
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