I am looking for an anti-mouse MOMA-1 (CD169) antibody that works on PFA/FFPE fixed tissue for IF. Does anyone have suggestions?
Thanks!
I'm using a fluorescence plate reader to titrate FITC and TRITC. Should I set the plate reader to read at the excitation and emission levels for each of the fluorophores?
03 April 2019 5,701 2 View
I am looking for a protocol to expand and maintain quiescent human hepatic stellate cells. I have a batch that I'm nervous about expanding, because I want to make sure they stay quiescent. I have...
10 November 2018 4,343 2 View
Hello, I would like to assess peptide levels in cortical tissues. I have samples that are currently in TRIzol, and I am wondering if there is a way to use these samples in an ELISA. Thanks!
06 July 2015 2,231 4 View
Hello, I'm starting to do mouse surgeries that are several hours long. I've been looking into feedback-controlled thermoregulator units, but they are quite expensive (~ $2,000). I'm interested...
31 December 2014 7,582 7 View
I inherited a set of primers for rat COX2 and thought I'd see if it worked on mouse samples. I used Primer BLAST to put in the sequences, specified mouse, and got back results indicating that my...
05 June 2014 2,811 5 View
I'm using NCBI's Primer-Blast to design primers for qPCR. When I get the results, is the first primer pair listed generally my best bet? I ask because I recently noticed that the first primer...
05 June 2014 2,872 8 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.
23 July 2024 4,649 2 View