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Questions related from Anne Tye
I'm using a fluorescence plate reader to titrate FITC and TRITC. Should I set the plate reader to read at the excitation and emission levels for each of the fluorophores?
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I am looking for a protocol to expand and maintain quiescent human hepatic stellate cells. I have a batch that I'm nervous about expanding, because I want to make sure they stay quiescent. I have...
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Hello, I'm starting to do mouse surgeries that are several hours long. I've been looking into feedback-controlled thermoregulator units, but they are quite expensive (~ $2,000). I'm interested...
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I inherited a set of primers for rat COX2 and thought I'd see if it worked on mouse samples. I used Primer BLAST to put in the sequences, specified mouse, and got back results indicating that my...
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I'm using NCBI's Primer-Blast to design primers for qPCR. When I get the results, is the first primer pair listed generally my best bet? I ask because I recently noticed that the first primer...
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