Keeping primary human hepatic stellate cells quiescent?

I am looking for a protocol to expand and maintain quiescent human hepatic stellate cells. I have a batch that I'm nervous about expanding, because I want to make sure they stay quiescent. I have...

10 November 2018 4,313 2 View

Anti-mouse MOMA-1/CD169 IHC-P antibody for IF?

I am looking for an anti-mouse MOMA-1 (CD169) antibody that works on PFA/FFPE fixed tissue for IF. Does anyone have suggestions? Thanks!

08 September 2018 1,168 0 View

Is it possible to run an ELISA on tissues that were previously homogenized in TRIzol?

06 July 2015 2,193 4 View

What to use for thermoregulation during mouse surgery?

31 December 2014 7,555 7 View

Given a pair of primer sequences, how can I determine what gene will be amplified?

I inherited a set of primers for rat COX2 and thought I'd see if it worked on mouse samples. I used Primer BLAST to put in the sequences, specified mouse, and got back results indicating that my...

05 June 2014 2,779 5 View

What is the best online PCR primer design?

I'm using NCBI's Primer-Blast to design primers for qPCR. When I get the results, is the first primer pair listed generally my best bet? I ask because I recently noticed that the first primer...

05 June 2014 2,835 8 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View