Does someone have experience with an anti-human CD4 antibody that works well in IHC (paraffin)?
Though most of these are for FC, you might find pAbmAbs reviews worth a look: https://pabmabs.com/?cat=388
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05 July 2024 2,107 1 View
It is required to make a precursor gel for a SAPO-11 Zeolite, the compounds are the following: * As a source of Al: Aluminum Isopropoxide (98%) Formula: C9 H21 O3 Al PM: 204.24 g/mol Density:...
01 July 2024 617 3 View
Do you think the use of virtual reality with dyslexia students is good?
23 June 2024 8,437 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Hello, Can somebody please give me some reference to a paper or book where it is explained how to condensate: * from a Q8 serendipity FE to a Q4 FE with drilling DOFs (16 DOFs to 12 DOFs). *...
05 June 2024 936 1 View
Hi everyone, I need help and yuoue experience!!! What kind of cell culture contamination is it? Video attached: The cells are lung cancer cells that are thawed from -80°C fridge and we known that...
04 June 2024 9,619 2 View
I am confused on how the sand content is calculated in aggregate size fractions separated by wet sieving, especially when the fractions are to be used for further analysis. If they are to be...
06 April 2024 5,884 1 View
Hello everyone, I am trying to do surface staining of a protein of interest in adherent cells for analysis in FACS. However, I am not getting what I am expecting, and I am wondering if something...
18 March 2024 6,258 2 View
La multiculturalidad suele influir en la eficacia de programas de intervención social. Algunos enfoques incorporan la sensibilidad cultural, participación comunitaria y la adaptación para abordar...
11 March 2024 8,028 1 View
Debido al avance de las tecnologías, hay varias apps para poder acceder a citas con los servicios sociales, al igual que facilitan el acceso a la información, asistencia y recursos entre otras cosas.
11 March 2024 7,486 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
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24 July 2024 1,559 1 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View