Hello everyone
I do not completely understand the theory of processing the data of flowcytometry DCFH-DA based ROS kit.
What do "Geo Mean" and "M2" refer to? Which one should I use as a ROS indicator?
Noting that they had given me conflicting results.
Any advice will be appreciated.
If you could tell specifics (any link, kit and abbreviations), the community would understand what you are talking about. In the current question, it is very difficult to understand.
Thank you Shin Murakami , I'll try to rephrase the questions and attach some figures
You can send me a snap of the plot or image you finally got. Then I can help you actually. What are the gatings and population name !
Parash Prasad
My question is what are these items, " All, M2, % gated, Mean, Geo. Mean"
At what point do we say the change is significant?
Sorry for this late answer. The all means the total population count. It could be singlet cell, doublet cell or debrie. Of that, we only take or gate the cells, that are singlet and measure the DCFDA flourescence. Now, as the unstaind cells also have some autofluorescence, we have to first measure, the autofluorescence amount. On that plot or graph, we place a gate which will take those cells only which have fluorescence value, higher than the unstained value... This population is M2 population. In this M2 populatio, now you chech either your treatment increased or decreased the mean fluorescence intensity of the cells.
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