If I inoculate the E. coli MG1655 in LB and incubate it on bench overnight, will the E. coli do anaerobic respiration or fementation? Since LB is a rich medium with all sorts of metabolites, it may have the electron acceptors for example, nitrate or nitrite. Anybody could help me with this question or direct me to the right literature? Thank you!
Under anaerobic conditions and in the absence of alternative electron acceptors Escherichia coli converts sugars to a mixture of products by fermentation. The major soluble products are acetate, ethanol, acetate and formate with smaller amounts of succinate. In addition the gaseous products hydrogen and carbon dioxide are produced in substantial amounts. The pathway generating fermentation products is branched and the flow down each branch is varied in response both to the pH of the culture medium and the nature of the fermentation substrate. In particular, the ratio of the various fermentation products is manipulated in order to balance the number of reducing equivalents generated during glycolytic breakdown of the substrate. The enzymes and corresponding genes involved in these fermentation pathways are described. The regulatory responses of these genes and enzymes are known but the details of the underlying regulatory mechanisms are still obscure. D.P.CLARK (1989): The fermentation pathways of Escherichia coli, FEMS MICROBIOL.REV. 5(2):223-234
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