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We are genome editing Clotridioides difficile via CRISPR system. However, we met some difficulties generating a clean knock out. The colonies after editing showed both WT and KO PCR bands as the...
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If I inoculate the E. coli MG1655 in LB and incubate it on bench overnight, will the E. coli do anaerobic respiration or fementation? Since LB is a rich medium with all sorts of metabolites, it...
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I am currently working with E. coli MG1655. I want to knock out the lacZ gene in the chromosome. Does anyone know a quick way to knock out this gene without using any phage? Thank you very much!
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