Python Code for Dynograph Identification?

Hi all researcher, I am looking for references of python code that could identify the dynograph card. The identification includes identifying the shape of the dynograph result. My goal is to make...

13 December 2020 2,649 4 View

Can you superimpose immunofluorescene images?

Hi All, I would like to know if there is a way to superimpose serial sections of tissues after doing immunofluorescence. The problem is I want to see the co-localization between few proteins and...

22 September 2020 1,124 3 View

International Journal on Emerging Technologies ISSN: 2249-3255 is a scopus indexed journal?

International Journal on Emerging Technologies ISSN: 2249-3255 is a scopus indexed journal?

05 August 2020 9,497 13 View

How is the co-expressed genes regulatory network plotted? Is there any Bioinformatics tool for the same?

Hi, I am working on molecular validation of co-expressed genes in Rice under moisture stress. I have a list of genes that are co-expressed in three rice varieties, under a particular set of...

12 July 2020 7,494 1 View

How to simulate Welding - Goldak Double ellipsoid heat source model in ABAQUS ?

I am working on simulating Welding using the Goldak Double ellipsoid heat source model in ABAQUS. But I am not sure how to define the heat source as mentioned in the equation and also defining the...

27 April 2020 4,624 1 View

Is it possible to change p_top_requested from 5000 to 1000 in WRF?

I use the GFS files as input in WRF, but I get an error when I try to change p_top_requested from 5000 to 1000 hPa. Kindly suggest.

04 December 2019 2,121 4 View

What are all the data's to be added for Plasma disruption prediction using machine learning ?

Thank you for spending your precious time: I am working on GOLEM TOKAMAK dataset for disruption prediction using machine learning and i found the below features...

28 January 2019 4,366 1 View

Anone facing phototoxicity in IF samples?

Hi, Iam doing IF in HeLa cells and facing a weird problem. I use the standard protocol and when I expose the cell to laser light in the 488 channel, all the fluorescence fades the instant its...

29 September 2018 2,510 3 View

How to create an attack for a wireless network in ns3?

To detect anomalies in wireless network we created a wireless network in ns3. Now we want to create attack for that network.

15 February 2018 2,739 3 View

How to represent western blot for paper ?

Hi, I am trying to represent my western blots for paper and have a clarification how to represent the Y axis in the graph. I normalized the test protein with actin and then divided my test protein...

27 September 2017 7,856 4 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

What is apparent Km Value of an Enzyme, Is it different from usual Km value and Why it is important?

It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.

27 September 2020 3,522 7 View

What is the difference between PCR and the initial steps (before SDS gel) of DNA sequencing? Which method is more effective?

I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.

25 September 2020 7,968 5 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,359 2 View

How strong is Flag tag binding to Anti-Flag antibody?

I am trying to plan an pulldown assay to find some prey protein for my bait protein. I am interested in using the flag tag because it is small and doesn't disturb structure and doesn't require...

11 December 2019 2,406 3 View

How to calculate the dosage of enzymes in wheat flour?

I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...

10 November 2019 3,608 1 View

Can I restrict the amount of one species when using KinTek for kinetic fitting?

I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an...

09 October 2019 3,835 4 View

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me. Does somebody have this plasmid?

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me, would be needed for my work. I would like to test the interaction between my labor's new...

05 June 2019 4,133 1 View

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08 January 2019 3,512 5 View

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31 December 2018 8,083 5 View