Asparaginase has significance in cancer therapy. What would be the best assay for enzyme asparaginase?
A rapid plate assay for screening l-asparaginase producing micro-organisms
R. Gulati, R. K. Saxena, R. GuptaArticle first published online: 31 OCT 2003
Try that one. I do not know if this can be applied to mammalaina cells.
Shirfrin, S., Parrott, C.L. and Luborsky, S.W. (1974) Journal of Biological Chemistry 249, 1335-1340, The assay is based on the nesslerization reaction. If only screening then the paper already mentioned will help.
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