Amplification of a gene in Colorectal cancer clinical samples?

28 August 2020 1 9K Report

Dear all, Recently we tried to compare mRNA expression levels of a gene via real-time PCR in 40 colorectal cancer clinical isolates and 10 normal mucosa samples.

Interestingly, a reasonable expression was determined in tumor samples (Cq: 25-31) while no amplification at all (No Cq) was observed for normal mucosa samples. All the samples were processed for RNA extraction, cDNA synthesis and confirmation for a reference gene at same time. Our equipment is also working fine considering its 96 wells.

Would like to hear expert opinion from this forum for possible reasons why a reasonable expression profile was there for all tumor samples, while no signal at all was observed for the normal mucosa samples in the performed real-time PCR experiment.

Pablo Hernández-Alonso

Dear Asim,

Your results are suggesting that the gene you are assessing is maybe not expressed in non-cancerous cells. Which is the gene you are evaluating? Maybe we can check in silico if it has been reported expressed or not.

KR.

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