when ampicillin powder is dissolved in water , the powder particles first stick together like a gum and then dissolve slowly unlike sodium chloride which dissolves unformy by fairly mixing in the solution. why is it so?
When ampicillin enters the water, its amino group -NH2 and the carboxyl group -COOH interact with water to form ionic aggregates -NH3+ and
-COO- that promote reaction between themselves (gum)...-NH3+ --OOC-...
If i want to test a particular compound/metabolite on my yeast strains ( which have been transformed with the desired disease plasmid and shows the phenotype) . Then what is the method that I...
06 July 2018 3,182 0 View
In plant pathology experiments generally disease severity measured in terms of grades of affected leaf area in relation to time is used for plotting the area under the disease progress...
05 June 2018 8,058 0 View
Which is a better one to suspend extracted DNA? TE buffer or ultrapure water?
05 June 2018 3,942 4 View
Can a leaf tissue be suspended in CTAB buffer until its DNA extraction? like for example if i go to a field and collect the leaf tissue and suspend the leaf tissues in CTAB in an eppendorf tube...
05 June 2018 1,717 1 View
IS CTAB buffer method of DNA extraction suitable for extracting insect DNA?
05 June 2018 8,074 2 View
my 100 bp and 1kb ladder are not separating properly in gel inspite of providing the optimum agarose concentration, voltage and running time. the individual size bands dont spatially separate...
05 June 2018 1,179 0 View
In what way does preheating of CTAB buffer prior to DNA extraction from leaf tissues improve the efficacy of DNA extraction or DNA recovery
05 June 2018 3,705 4 View
I have cloned my virus in puc 18 vector. Right now Iam maintaining the clones by repeated subculture on lb medium amended with ampicillin. Is it possible to maintain the clones in glycerol...
04 May 2018 3,604 2 View
plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?
03 April 2018 1,618 3 View
Is it possible to make a readymix of PCR reaction cocktail involving components like water, dntps, taq polymerase etc. and keep it under long term storage for future use or should we use the mix...
03 April 2018 9,933 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.
31 July 2024 10,109 3 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Some of the techniques I am familiar with are standard. I would like to know which of the two methods under study is preferable, as well as the cost and list of particles and devices needed in...
22 July 2024 1,921 3 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
Hi everyone, I experienced some moving particles in my mouse hippocampal slices. The media looks clear. Any idea what these moving particles might be? Thanks you!
22 July 2024 3,734 0 View
I want to make 100 ml of MWCNT/distilled water mixture at a volume fraction of % 0.1. MWCNT density is 2100 kg/m3. I will add gum arabic as surfactant. If I add surfactant at a 1:1 ratio, will the...
19 July 2024 8,494 0 View