Hi, i'm trying to isolate many fungal strains, but the strains are contaminated wiht bacteria; i get Gentamicin (VIAL OF 2 ML WITH 160 mg OF GENTAMICIN SULFATE) my question is... wich is the best concentration and how much quantity add in 500ml of Potato dextrose agar to avoid the bacteria growth???. Thanks
Hello, use Gentamicin (500 mcg/ml) and Chromphemnicol (50 mcg/ml) in media for better results.
Good luck!
I've used an antibiotic cocktail instead with good results: 15 mg/L streptomycin sulfate, 15 mg/L gentamicin sulfate and 12 mg/L tetracycline hydrochloride.
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Oadi Matny How many amount of Gentamicin (500 mcg/ml) and Chromphemnicol (50 mcg/ml) per liter of PDA did you use?
Karen A. Darbinyan How many amount of Gentamicin 10 mg/L per liter of PDA did you use?
Xabier Vázquez-Campos How many amount of 15 mg/L streptomycin sulfate, 15 mg/L gentamicin sulfate and 12 mg/L tetracycline hydrochloride. per liter of PDA did you use?
Hello mcg/ml is same like mg/l so
Use
Gentamicin (500 mg/l) and Chromphemnicol (50 mg/l)
Marco Antonio Tucuch Pérez those are the final concentration in PDA
I agerr with Karen A. Darbinyan
use
Gentamicin (500 mg/l) and Chromphemnicol (50 mg/l)
Hi, have you please any reference for the Gentamicin concentration 160mg?
Thank you
You can use Gentamicin with a percent of 500 mg/l or Chromphemnicol with a percent of 50 mg/l by adding any one of them to the media to prevent the contamination with colonies of bacteria in Petri dishes.Also you can use Streptomycin sulfate or Tetracycline hydrochloride.
I can say that Gentamicin is effective against Gram negative bacteria only, but Chloramphenicol is effective against both of Gram positive and Gram negative bacteria, therefore I prefer using it with fungal culture to prevent all types of bacteria.
Dear, Marco Antonio Tucuch Pérez
50 mcg/ml of Chloramphenicol is equal to 50 mg/l, and 500 mcg/ml of Gentamicin is equal to 500 mg/l, because the mg =1000 mcg and the 1L= 1000 ml.
With best regards.
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