But if I am using using crude enzyme instead of purified cellulase enzyme what should be the ratio?

Lai, the question is not sufficient to give any appropriate answer to it. The type of cellulase enzyme and property of the cellulose also affect the enzymatic hydrolysis al lot.

Agree with Manas Swain

Dear All,

Great day. I m really sorry for late respond.

Thank you all the answers provided.

Let take example:

cellulase activity was determined at 70 FPU/ml.

(followed Ghose's Method, 1987)

Q1.

2% substrate in 30ml working volume for 5 days (120 h). 30 FPU would be used,

then how many (ml / ul) of cellulase enzyme should add into above conditions (2% in 30ml)? Could show the way to calculate?

Q2.

If the B-glucosidase activity was determined at 2.8 U/ml (using 1% salicin as substrate).

How many (ml / ul) of B-glucosidase enzyme should add into above conditions (2% in 30 ml)

If cellulase to B-glucosidase ratio is 5:1

If cellulase to B-glucosidase ratio is 1:5

Could show the way to calculate?

I am novice in ligocellulose enzymatic study. Hence, seeking technical advice and your expertise.

Hope can hear from you guys soon.

Thank you in advance.