How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

How to extract trypsin inhibitor from fermented samples ?

Hi. I need help to measure trypsin inhibitor activity in fermented soy and fermented oat samples. The problem is my samples were mixed with water with ratio 1:3 and 1:2 respectively, for...

13 December 2020 6,882 3 View

Is there any way to revive strains after a year stored in 4 degree refrigerator?

The strain is Candida tropicalis. It has been stored in 4 degree refrigerator for a year. Now we want to see if it is possible to revive the strain and make it function again. I tried to culture...

10 November 2020 5,650 9 View

What does over foaming correspond to in E.coli fed-batch fermentation?! How does foaming relate to cells metabolic status?

I'm currently experiencing overfoaming in my E.coli culture. My cells are used to produce a recombinant protein. The basic cell strain is BL-21 DE3. After changing my carbon source brand i am...

06 October 2020 3,946 3 View

How to measure the growth and enzyme production profile over the fermentation period?

01 October 2020 9,300 4 View

Why Lactobacillus acidophillus is sensitive to Vancomycin but L. rhamnosus and casei are intrinsically resistant?

Does it have, anything to do with their fermentation status? I know that in several species of LAB, the terminal d-alanine residue is replaced by d-lactate or d-serine in the muramylpentapeptide,...

19 September 2020 3,242 2 View

Effective method for removing precipitation of CSL for biomass production? ( I centrifuged CSL but after autoclave precipitation was formed)?

We are going to use CSL as a cost-effective nitrogen source for Lactobacillus fermentation in pH 6.3. We have examined the acid treatment method to lower the pH of CSL to 3.5; after that, we...

12 September 2020 6,784 2 View

How to Identify and Estimate the Lactic acid from the Microbial fermented broth other than HPLC using Simple methods?

How to Identify and Estimate the Lactic acid from the Microbial fermented broth other than HPLC using Simple methods? if possible such as 1. Calorimetric or Spectroscopic methods and much more

26 August 2020 349 10 View

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08 August 2020 1,314 3 View

How giant pandas get all their nutrition on their bamboo-only diet?

Giant pandas have a carnivorous digestive system, yet they eat nothing but bamboo all day and every day. They have a simple stomach, not like the ruminants that have four chambered stomach for...

05 August 2020 8,238 4 View