Is it possible to get AMF spores (just for counting purpose) from soil samples stored for more 2 months under room temperature?or for more than a year at +4?
actually sub-zero temperature is required. There is a high chance of sporulation at room temperature.
Please consider these works, hope you find some clues...
- Mycorrhiza. 2007 Sep;17(6):519-26. Epub 2007 Mar 7.
Sporulation and diversity of arbuscular mycorrhizal fungi in Brazil Pine in the field and in the greenhouse.
Abstract
The aim of this work was to assess the sporulation and diversity of arbuscular mycorrhizal fungi (AMF) at different forest sites with Araucaria angustifolia (Bert.) O. Ktze. (Brazil Pine). In addition, a greenhouse experiment was carried out to test the use of traditional trap plants (maize + peanut) or A. angustifolia to estimate the diversity of AMF at each site. Soil samples were taken in two State Parks at southwestern Brazil: Campos do Jordão (Parque Estadual de Campos do Jordão [PECJ]) and Apiaí (Parque Estadual Turístico do Alto Ribeira [PETAR]), São Paulo State, in sites of either native or replanted forest. In PECJ, an extra site of replanted forest that was impacted by accidental fire and is now in a state of recuperation was also sampled. The spore densities and their morphological identification were compiled at each site. In the greenhouse, soil samples from each site were used as inoculum to promote spore multiplication on maize + peanut or A. angustifolia grown on a sandy, low-fertility substrate. Plants were harvested, respectively, after 4 months or 1 year of growth and assessed for mycorrhizal root colonization. Spore counts and identification were also performed in the substrate, after the harvest of plants. Twenty-five taxa were identified considering all sites. Species richness and diversity were greater in native forest areas, being Acaulospora, the genus with the most species. Differences in number of spores, diversity, and richness were found at the different sites of each State Park. Differences were also found when maize + peanut or A. angustifolia were used as trap plants. The traditional methodology using trap plants seems to underestimate the diversity of the AMF. The use of A. angustifolia as trap plant showed similar species richness to the field in PECJ, but the identified species were not necessarily the same. Nevertheless, for PETAR, both A. angustifolia and maize + peanut underestimated the species richness. Because the AMF sporulation can be affected by many conditions, it is impossible to draw detailed conclusions from this kind of survey. More precise experiments have to be set up to isolate the different factors that modulate the ecophysiological interactions between host plant and endophyte.
PMID: 17342509 DOI: 10.1007/s00572-007-0124-7
In my opinion we can count AMF spores from soil stored at room temp or from soil stored at 4.C which canbe counted by staining. For it the plant roots infected with AMF should be macerated with soil before counting of fungi.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany