Dear community,
I am planing to transplant human cells into a mouse disease model and would require the use of immunosuppressive drugs. Usually I read in publications that people daily i.p. inject the drugs or rely on mouse with a defective immune system.
Since my time line is pretty long, we have a rather large cohort of animals that will undergo a lot of behavioral testings, I would like to avoid daily injections.
Do you know if there are substances, slow release systems or other alternatives that are longer lasting in the field of immunosupression?
Unfortunately, the use of conventional genetically modified models is not a good option for us because we do not want to alter the inflammatory responses in the disease model before implanting the cells. Or are you aware of reliable inducible immunosupressed genetic mouse models?
I would be very thankful for any suggestions!
Many thanks!
Ruslan
Dear multiple blood transfusion can be an alternative to immunosuppressive drugs.
Alzet pumps are a good way to avoid daily injections: http://www.alzet.com
However, immunosuppression alone may not solve all the problems of rejection in a xeno-transplant situation. There are many reasons human cells are rejected by mice.
What about use humanized mice models? You are not going to have graft vs host disease and at same time immune system and immune response.
Miguel Gallardo Many thanks for the reply! Are there any models you would particulartly recommend?
And do you have experience if these humanized mice models do not require any immunesupression? (since there are different human cell sources of iPSCs etc.)
It depends on your disease, experimental design etc, but for instance, in hematology, BRGS (BALB/cRAG/IL2/SIRPalpha) humanized mice model.
Alzet pumps can deliver your drug, but I think your problems won't be solved with that. GVHD may still occur, as well as rejection.
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