CF11 cellulose columns have been used to filter white blood cells out of human blood for subsequent studies on malaria parasites. Now the producer of CF11, Whatman, does no longer produce it. Does anyone has experience with alternatives? The stage distribution of the parasites should not be distorted.
We are using a cellulose from Sigma (cat number C6288). We are achieving the same result as with CF11 columns.
Hi Stefanie!
Thanks a lot! I will try it out and let you know how it goes once I have results.
Wow! Thanks Stefanie, That's a great tip!! We will try it! Is this published anywhere?
An update to the original publication, describing the use of Sigma 6288 instead of CF11, has been published: http://www.malariajournal.com/content/11/1/41/comments#935696
I used to use CF 11 cellulose powder to separate dsRNA elements from total nucleic acid preparation. In 15-16% ethanol concentration, CF 11 specifically binds dsRNA fractions. As Whatman stopped producing this product, I am wondering what would be the alternative for CF 11. One of my friends suggested me to use Sigmacell Cellulose (Type 101, Highly purified, fibers), cat no. S6790-100G. Do you guys have any idea about this Type 101 cellulose? What you think, would it serve my dsRNA separation purpose? Please suggest. Thank you very much for your time and consideration.
Dear All,
I am thinking to use Whatman CF11 cellulose powder columns for purifying P. berghei parasites. I haven’t done this kind of work before. Can anyone suggest which one is better among Whatman CF11 cellulose powder columns and Plasmodipure ?
Looks like CF11 cellulose powder columns discontinued. Not very sure which one should be picked.
Any suggestions from experience user will be highly appreciated
Thanks
Deepak
Hi Deepak,
yes, CF11 was discontinued and to the best of my knowledge is not available any more. As by the answers above, you can use Sigma C6288 instead, it worked well for me.
Plasmodipur enriched for certain stages of the parasite, thus it depends on your question whether it is suitable for you. It is also much more expensive than Sigma C6288.
Cheers,
Cristian
Hi Cristian
Thank you very much for the kind information. I will use it and let you know my experience. Thank you again for the great help.
Kind regards,
Deepak
A more efficient alternative to CF11 is the MN2100ff cellulose powder:
https://www.malariagen.net/news-events/news-article/online-protocol-leucocyte-depletion-using-mn2100ff-cellulose-columns
Very cheap. Here is the video showing how to prepare your columns:
https://www.youtube.com/watch?v=JGqVDYC-4_U
Antoine
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