Subha Das

5 Questions 27 Answers 0 Followers

Questions related from Subha Das

Any suggestions for an alternative to Rosetta™ 2(DE3)pLysS Cells?

Hi All, I wanted to purchase Rosetta™ 2(DE3)pLysS Cells (Product No# 71403-M )from Merck but I was told that this product has been discontinued. In the circumstances, could you kindly...

19 August 2024 2,916 8 View

What is the best protocol for Viroid isolation from plants?

I am looking for a protocol to isolate unknown viroids from plant samples. Just wondering if there is any kit available for this

03 July 2019 4,157 9 View

Would like to know about major fungal diseases of Mangos in India?

Dear Dr. Kulkarni, At first, I would like to introduce myself. I am Subha Das, presently based in Okayama University (IPSR), Japan, working as a JSPS postdoctoral fellow since May 2018. I mainly...

23 April 2019 3,220 9 View

Alternatives to CF 11 Cellulose powder for dsRNA extraction?

Whatman CF 11 cellulose powder (Cat. No. 4021-050) has been used to separate dsRNA elements from ssRNA, ssDNA and dsDNA. Column chromatography under 15% ethanol concentration, CF 11 cellulose...

19 February 2015 5,342 5 View

Can I autoclave CF-11 cellulose powder to be used for double-stranded RNA (dsRNA) affinity chromatography?

For cellulose affinity chromatography-based specific isolation of dsRNA, researchers use CF-11 cellulose powder. CF-11 cellulose, in the presence of 15-16% enthanol concentration, specifically...

19 January 2015 4,592 6 View