Hi, everyone. In our lab we are looking for a cheaper alternative to the Qiagen kit, but with similar quality. Any suggestions?
Please (for Arabidopsis), what could be a good Lumens and color range (Kelvin) range for full spectrum LED lamp tubes size T8 (120cm) for each shelve measuring 130x50 cm (length x width) and 60 cm...
11 July 2024 6,078 1 View
Hello, I am a doctoral student in Brazil. I need to perform the transfer to check for fibronectin immunodetection (250 kDa). Our transfer buffer was heating up too much; I even changed the ice...
25 April 2024 1,700 1 View
I'm trying to use aspen to simulate the reactive distillation of butanol and acrylic acid to butyl acrylate using a RadFrac unit and LHHW kinetics. As pointed out by many other posts, RadFrac does...
26 February 2024 7,018 1 View
Is it measured from the apical parts to the center of the microalgae ? or do you have another ways to measured it? I attach an image to explain thanks
12 February 2024 6,924 3 View
I want to find an alternative method since we do not have a distilling equipment with vacuum in the lab. Please suggest other methods to remove hydroquinone?
06 February 2024 1,457 0 View
The signs of times: Choreographing life on TikTok? This presentation departs from longitudinal research with families (2017-2022), with diverse ways of living and different demographics, touched...
24 January 2024 9,807 0 View
I have prepared a liquid medium for fermentation with kraft lignin in water, but the lignin was not completely dissolved. After fermentation, I am wondering about the best way to filter out the...
04 January 2024 7,479 1 View
I need to prepare a liquid culture medium for enzyme production. Kraft lignin will be the substrate in the medium. What would be the best solvent for this?
26 November 2023 7,130 2 View
I need help with a PCR. I have the following primers mecA-F 5′AAAATCGATGGTAAAGGTTGG-3′ / mecA-R 5′-AGTTCTGGCACTACCGGATTTTGC-3′. I made an initial PCR with 12.5 of GoTaq Green Master Mix, 2x, 0.8...
05 October 2023 7,228 2 View
We can purchase poly-lysine coated coverslip but they have an expire date. Could we coat again with poly-lysine some expired coverslips?
28 September 2023 976 1 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...
24 July 2024 9,688 0 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...
14 July 2024 7,475 1 View
I have DNA samples (extracted by using Qiagen kiit), the 260/280 ratio is consistence 2.1.. for most of the samples. Can I proceed for the whole genome sequencing with these values? NGS.
07 July 2024 3,751 4 View
I am trying to extract DNA from the cyanobacteria Microcystis aeruginosa using the Dynabeads DNA DIRECT Universal kit for the purpose of on-site detection. Yield is acceptable at ~100-500 ng/uL,...
01 July 2024 10,003 1 View
Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...
18 June 2024 6,854 3 View
I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....
27 May 2024 1,303 1 View