Hi all,
due to the shortage of Matrigel, we would like to find suitable alternatives for the culture of iPSCs.
We have tried Geltrex without success. Next, we will try Vitronectin. Some colleagues have tried Synthemax but did not like it.
By browsing other topics on Research Gate, I read about Jellagen and plant cellulose -based hydrogels (GrowDex-T) and I'd like to know if anyone tried these with iPSC specifically.
I also read about Cell Basement Membrane from ATCC, any opinion on this one?
I will be grateful for any help and tips!
Hi,
I usually use StemFit AK02N and iMatrix-511 Silk based on laminin 511 fragment.
https://www.ajitrade.com/stemfit/products/ak02n_03n.html
https://matrixome.co.jp/en/products/imatrix-511silk
These work well in our lab.
I have used Geltrex for iPSC culture (both 2D and 3D culture) and they work similar to Matrigel.
I use iMatrix-511 and have had no issues, and now I prefer it. I use it how stated within pre-mixing method within the manual.
Pluripotent stem cells proliferate and retain their pluripotency well in nanofibrillar cellulose hydrogel, GrowDex. It is animal-free and well-defined matrix for 3D culture of various cells, including hESC and hiPSC.
Please see the great work by Lou et al. with stem cells and GrowDex.
Article The Use of Nanofibrillar Cellulose Hydrogel As a Flexible Th...
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