Does anyone have an old digital 'whole slide' scanner that they can donate to a good cause?

New transplant programme starting in a small African nation, but they will need support and training in the examination of biopsies. A digital 'whole slide' scanner would allow virtual biopsy...

27 November 2019 9,900 1 View

Is transgender a feeling or a desire?

I encounter difficulty in getting the sense of transgender. If it is feeling how does it develop? If it is desire what motivates it? It is important that I should have knowledge on this phenomena...

23 July 2019 5,137 4 View

What are possible substitutes for P2O5 as a desiccant?

I've been using phosphorus pentoxide as the desiccant while drying my enzymes; however, our stockroom has now run out of the chemical. What are other chemicals that can be used as desiccants that...

12 January 2018 8,072 4 View

How to calculate the average correlation across samples in a meta analysis?

Dear researchers, I am conducting a meta analysis which analyses the correlations between personal values and different well-being scales across different studies and by that across different...

26 December 2017 748 6 View

Have a PCR product obtained with degenerate primers, need to clone. Interesed in one band specifically, how do I choose the ratio product:vector?

I am using degenerate primers to amplify a family of genes. My results in an agarose gel are 6 bands (862pb, 632pb, 408pb, 335pb and 226pb). The bands of interest are 408pb and 335pb. What...

27 November 2017 7,346 3 View

Any one tell me. what is the concentration of below the sample buffer 1x or 10x?

14 July 2017 9,607 3 View

Do I need longitudinal data for path analysis?

Dear researchers, I plan to investigate possible causalities of certain psychological constructs and variables like Engagement, Goal attainment, Well-being... Based on correlation analyses and...

09 November 2016 3,681 2 View

What's the most recent Portraits Values Questionnaire version (PVQ-RR?)?

I would appreciate it and be very happy if anybody could tell me which is the most recent Portraits Value Questionnaire (PVQ) based on Schwartz et al. 2012 with 57 items (not PVQ21 or PVQ40)?...

20 March 2016 2,614 8 View

Almost all soil analyses carried in the laboratory are done on 2 mm sieved soil. Can someone please explain why?

04 July 2014 4,221 14 View

In a potted maize experiment, what is the appropriate way to collect leachate? What is the appropriate leachate storage temperature and duration?

l am about to carry out a pot experiment and l need to know the appropriate way to collect leachate, its storage temperature and duration and the appropriate leachate sampling interval/period. l...

10 March 2014 9,902 10 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View