I am now doing a study that relate to bone regeneration. I want to ensure the procedure for the ALP assay which are faster and cheaper. I did review some previous study on ALP Assay. Some of study are using Kit for the assay which is easier, however that price for the Kit is quite expensive. Meanwhile , the other study also shows ALP assay by doing it manually. I want think to do it manually, but i am afraid there will be a step that i will misconduct during the experiment. So, here i want to know which of the method is preferable for me to proceed the test. Is anyone are familiar with ALP assay for bone regeneration (osteoblast).
Thank you.
Thank you for the explanation Amin. I would proceed with using the Kit then to ensure that the outcome is really accurate.
I would prefer the Kit from Sigma. It is possible to refill the Citrat solution in the Kit, because from my experiences the staining solutions will last very long, but the citrate solution not. This would be a compromiss.
Can anyone suggest the Catalog number for the kit from sigma? Anyone suggest a cell line or this? I'm looking to set up a model for bone health, but I can't get my hands on Saos-2 cells.
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