Sarah Ashby

3 Questions 3 Answers 0 Followers

Questions related from Sarah Ashby

How to link the cysteine group of a protein to a carboxyl bead?

Hi, I have carboxylated beads and I want to covalently attach a protein with the thiol group of a cysteine. Can I use SMCC chemistry for this? Are there linkers that will make this easier for me?

20 January 2022 9,461 3 View

Why does Sheep Monoclonal Antibody Fails to Covalently Couple to 200 nm Carboxylated Beads?

Hi, I have performed several conjugations with different antibodies (Human IgG, Mouse CRP) to 200 nm carboxylated beads using two-step EDAC/Sulfo-NHS method. The protocol, method and materials...

05 July 2021 6,411 4 View

Any tips for disassociating a small molecule ligand from a binding protein in the presence of antibodies?

Hi, I am running an assay that requires the disassociation of a small molecule from an albumin binding protein. The disassociation should occur in the presence of antibodies, so it can't be too...

05 June 2021 9,621 2 View