Does Alizarin staining help in the skeletal study of a fish. Is it a viable technique? Is the technique only applicable to thin fishes or small fishes or fish spawn, fry, fingerling?
Yes, alizarin red does a great job of staining bones in fish of all sizes. I've attached some papers of colleagues that employ the technique (Willacker et al., 2010 and Minicozzi et al., 2019) and one of the original papers that describes the protocol (Song and Parenti, 1995).
Hi Danielle,
Thank you very much for your quick response. The links of the documents you sent are very useful.
Best regards.
mostafa
Mostafa Hossain sir,
Thank you for putting the issue up front! I think there is huge scope to work on the matter.
In a fish taxonomy study of the family Trichonotidae, I attempted this and stuck there owing to lack to strong stereomicroscopes. Clearing and staining is relatively easy requiring strong ethanol, trypsin, alcian blue (for cartilage!) and alizarin red (for bones!)!
Please check the videos below, might interest you!
https://www.youtube.com/watch?v=yiF0wZ86ec8&t=29s
https://www.youtube.com/watch?v=haopSRCuPdo
https://www.youtube.com/watch?v=Vqky9bi-Cl4&t=42s
And, please let me know whether you have any plan for a similar work. Will gladly volunteer!
Regards,
Akash
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