I have added different number of cells (raji cell line) to wells of adjacent columns of 96 well plate starting from 3000 to 90000 cells. But the reading of all wells at 570 is same. For reference please see screenshot attached.
Potentially you are saturating your assay.
@ Robert , Thank you.
Please guide How to troubleshoot?
Try using a higher dilution factor or adding more resazurin.
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