AI Systems Regulation

01 January 1970 0 5K Report

According to Jacob Nagler, the only option to regulate AI systems is through AI monitoring systems.

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Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

How to simulate a metalens with backward propagating source using a Lumerical FDTD?

Dear Sir/Madam, I am currently working on a beam shaping problem and have referenced the Metalens example provided on the Ansys Lumerical website. In that example, a forward propagating plane wave...

14 June 2024 4,333 0 View

Do you know a partner, friend, colleague, or someone else who was a Canadian gay (male) nurse who cared for PLWH during the HIV/AIDS pandemic?

My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...

10 June 2024 6,253 0 View

Protocol for Coating Plates with Collagen?

Does anyone have guidance on coating 96-well plates with collagen-I? Looking for a protocol. We are using Corning® Collagen I, Rat Tail, 100 mg.

22 April 2024 6,835 1 View

How do I determine and what radical is this in this EPR graph (DMPO)?

So I'm trying to determine what radical this is captured with DMPO. How do I determine and calculate what radical this is? Thanks!!

06 April 2024 7,084 3 View

Is it required to send interview transcriptions for publishing qualitative research in a journal as part of data availability?

As someone engaging in ethnographic research, are we expected to disclose transcribed data to the journal where we would like publish an article?

03 March 2024 3,653 3 View

Which Promotor Sites can I use for In Vitro Transcription?

Our lab is trying to use in vitro transcription to create mRNA of our inserted on a pcDNA 3.4 TOPO plasmid. I noticed it does not have a T7 promotor sequence. Are there other available promotors...

26 February 2024 1,852 1 View

How should one teach ETHICS to the new generation of college students in 2024?

More and more, teaching ETHICS has become an important part of the college curriculum, but teaching it has not always been easy or up to part with the generational changes in the student...

02 January 2024 7,862 6 View

Buffering pH of DNA Binding Solutions for Silica Columns?

I am trying to prepare some custom DNA binding solutions for purifying DNA with silica columns. I have prepared one with 4 M guanidine thiocyanate, 30% isopropanol, and 10 mM Phosphate buffer, pH...

03 December 2023 2,197 0 View

How do we design a three level dimensions of organizational ambidexterity?

I am currently exploring on the concept of "Organizational Ambidexterity". I would love you give me an insight on the way to design its dimensions in a three level scale, where it will depict its...

27 October 2023 3,058 3 View

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations?

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...

17 July 2024 3,255 4 View

Consciousness and the neocortex are efficient users of energy?

It is well known that the larger the organism, the less energy consumed per unit mass (Delong et al. 2010; Moses et al. 2016; Wells 2007). This is known as Kleiber’s Law, whereby the energy...

06 July 2024 8,736 0 View

How can I better show my data?

I am validating the expression of several genes by qPCR to confirm transcriptome analysis. After many inconclusive runs, I realised my housekeeping gene (GAPDH) was downregulated in my...

27 June 2024 7,318 3 View

International Maritime Law?

International maritime law, also known as admiralty law, is a body of laws, conventions, and treaties that govern private maritime business and other nautical matters, such as shipping or offenses...

08 June 2024 4,794 0 View

Stress-related gene regulation through harvesting plants?

For my doctoral thesis I would like to carry out a gene expression analysis in the plant Melissa officinalis. For this purpose, I will examine various genotypes that are located in a field that is...

28 May 2024 5,833 1 View

Can you explain the significance of temperature regulation in pediatric anesthesia?

Temperature regulation is of utmost significance in pediatric anesthesia due to several reasons:

11 April 2024 2,413 1 View

High mRNA expression versus low protein expression?

I tested gene expression by RT PCR followed by Western blotting to test protein expression. I get an inverse correlation with up-regulation at mRNA level and down-regulation at the protein level....

05 April 2024 4,679 6 View

What it REPRINTS ?

"The BERQ can be used for free for research purposes. Please mail the authors to request reprints of the questionnaire." pls help me, i dont understand what is REPRINT while it FOR FREE for...

26 March 2024 6,904 0 View

Is it possible to see downregulation of a gene (Ki67) on RT-qPCR while seeing an upregulation on immunofluorescence staining?

Hi everyone, I performed an immunofluorescence (IF) staining for Ki67 using a validated, specific Ki67 antibody (Dako) and I see a clear upregulation of the protein expression compared to my...

14 March 2024 1,516 0 View

What is the ideal concentration of the MCP protein to add, in order to regulate the Tet ON\OFF system?

I want to regulate the expression of some genes that are under the Tet regulation in transfected U20S cells, By adding a purified MCP protein. what is the ideal concentration to start with? and...

14 February 2024 4,297 0 View