After transformation of ligation mixture in DH5 alpha cells, what type of colonies can we expect?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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fatal error

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fatal error

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Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

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Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

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Sameh E. Mohamed

This is strictly dependent on the expression vector you are using to transform your target DNA in E. coli DH5 alpha. For example, if you are using Blue/White screening method, then you can see blue colonies (transformed with native intact bluescript vector) and white colonies (transformed with the modified vector encoding your target gene). Other colonies without vector will die as you add an antibiotic (for example Erythromycin; Erm) to the LB media.