How can we maintain protein stability in its native conformation?

I am trying to show an interaction of a protein with RNA in EMSA, on the first day of purification it is showing good interaction in the sense that we are getting clear band, after 2 to 3 days we...

09 October 2014 3,348 9 View

What happens if we run SDS PAGE with a 5x tris glycine running buffer?

Will it create any problem for the bands?

04 May 2014 5,753 2 View

Is there any difference in gene amplification when it is present in the genome or in vector?

Sometimes I am getting the amplification when it is as cDNA, but by taking that amplified fragment and cloning into a vector it is not amplifying. What could be possible reasons for this?

03 April 2014 1,248 8 View

Is there any amplification difference if we are using PCR reaction mixtures of 20 & 50 microliters?

I am getting amplification with a 20 microliter reaction but not with 50.

02 March 2014 7,628 9 View

Is there any alternative method for touchdown PCR?

For the amplification of nrfa gene touchdown PCR is preferred. But we are facing problem with it for organism. Is there any other method of PCR for it?

01 February 2014 10,014 10 View

Even though HIV has high mutation rates, can't we prepare antibodies or vaccines by using capsid proteins?

This may be an unmatured question but I have had this doubt from my college days.

01 February 2014 9,755 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

No title

02 March 2021 3,060 3 View