M Ch Sekhar Srikakolapu

7 Questions 15 Answers 0 Followers

Questions related from M Ch Sekhar Srikakolapu

How can we maintain protein stability in its native conformation?

I am trying to show an interaction of a protein with RNA in EMSA, on the first day of purification it is showing good interaction in the sense that we are getting clear band, after 2 to 3 days we...

10 October 2014 3,388 9 View

What happens if we run SDS PAGE with a 5x tris glycine running buffer?

Will it create any problem for the bands?

05 May 2014 5,790 2 View

After transformation of ligation mixture in DH5 alpha cells, what type of colonies can we expect?

What kind of morphology can we expect? I got white colonies instead of transparent colonies. What can we infer from them?

04 April 2014 2,446 2 View

Is there any difference in gene amplification when it is present in the genome or in vector?

Sometimes I am getting the amplification when it is as cDNA, but by taking that amplified fragment and cloning into a vector it is not amplifying. What could be possible reasons for this?

04 April 2014 1,286 8 View

Is there any amplification difference if we are using PCR reaction mixtures of 20 & 50 microliters?

I am getting amplification with a 20 microliter reaction but not with 50.

03 March 2014 7,677 9 View

Is there any alternative method for touchdown PCR?

For the amplification of nrfa gene touchdown PCR is preferred. But we are facing problem with it for organism. Is there any other method of PCR for it?

02 February 2014 10,047 10 View

Even though HIV has high mutation rates, can't we prepare antibodies or vaccines by using capsid proteins?

This may be an unmatured question but I have had this doubt from my college days.

02 February 2014 9,790 2 View