After studying multi-component systems, can we use spectrophotometer to predict the structural changes at the time of interactions?

More Shilpi Vats's questions See All

How can I compute the distribution of dipole moment as a function of the angles between the dipole vector and solid surface through Lammps command?

I want to plot the distribution curve of dipole moment as a function of angles between dipole vector and solid surface in the presence of external electric field. My system is consisting a...

17 January 2021 1,508 4 View

How to make different assay components (like salt, buffer, glycerol etc) while working with RNA substrates?

Basically, I wanted to ask if we should prepare these components in RNAse free water and use directly or should we prepare them first and then autoclave them ? If we autoclave, wont it chnage the...

04 December 2020 4,588 2 View

Why Mrbayes does not produce .con output file?

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16 November 2020 3,339 1 View

Can anyone tell me what to do if the D-spacing vs Sin2(psi) Curve is not linear fitting in Residual stress measrunment by XRD?

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31 August 2020 2,477 3 View

How can I calculate the outlet concentration of a tracer injected from inlet using dpm particle tracking in Fluent?

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28 August 2020 9,962 3 View

How to add new mixture template in species transport model in Ansys Fluent?

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25 August 2020 6,340 2 View

If antibody of an unknown protein is provided then how molecular weight of that protein will be find out ?

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22 July 2020 7,351 2 View

How can I calculate the area of the the breakthrough curve using Origin software?

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01 February 2020 6,956 6 View

Absence of Path Coefficients and Estimates in Amos Output?

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11 August 2019 9,635 1 View

Does Ph plays any role in high temperature chemistry?

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21 March 2019 5,133 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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02 March 2021 5,785 3 View

Can I measure absorbance efficiency with wavelength in a spectrophotometer?

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16 February 2021 7,515 5 View

CAT assay in microplate?

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Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

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10 February 2021 6,221 1 View

Can anyone please suggest where I can find a reference for ReaxFF parameters for PTFE(Teflon) suitable for use in LAMMPS?

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How can I quantify the color of a solution?

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Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

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06 February 2021 9,370 7 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

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How to prepare an algal sample for FT-IR spectroscopy?

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30 January 2021 2,303 4 View

Is there an open source tool the measures the GRAVY index of a peptide incorporating the PTMs and unusual amino acids (e.g. hydroxyproline)?

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29 January 2021 8,300 4 View

Adam B Shapiro

It depends what biological molecules and what sort of structural changes. Unwinding of DNA is detectable by an increase in UV absorbance. Secondary structural changes in proteins can be detected as changes in the far-UV circular dichroism (CD) spectrum, which requires a CD spectrometer. Tertiary structure changes can sometimes be detected as changes in the near-UV CD spectrum, or in the intrinsic fluorescence spectrum using a spectrofluorometer. Fourier transform infrared spectroscopy (FT-IR) can also be used to investigate aspects of protein secondary and tertiary structure.

However, to get highly detailed information about structural changes, the best methods are multidimensional NMR and x-ray crystallography. Recent advances in cryo-electron microscopy are also making it possible to get detailed structural information about macromolecules by this method.