I am looking for a protocol to transfect adult rat myocytes with a good yield.
Thanks a lot!
You need to use a virus to transfect adult cardiomyocytes.
yes but unfortunately I don't have an adenovirus for the
moment .
The paper (attached) is a good resource for working with cardiomyocytes and provides alternate methods to viral transfection. That said, it does say that viral transfection provides the highest yields. Any how, I hope it helps.
thanks Daniel
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