Protocol for extracting DNA from colonies ?

31 December 2016 5,913 18 View

Knockout tested western blot antibody for mice TLR2?

Not all antibodies can be used for knockout confirmation since some WB antibodies can bind to both WT and knockout equally (since the antibody recognizes nonmutated regions from the knockout)....

07 August 2016 1,000 4 View

Intracellular Survival Assay - how can I lyse these cells for plating the lysates?

I was coculturing immortalized bone-marrow derived macropahges with Bifidobacteria. Any heads up on how to lyse these cells for plating the lysates to check bacterial persistence within these...

03 April 2016 7,583 2 View

Confirmatory test for bacteriocin producing lactic acid bacteria?

I have a bacteriocin producing lactic acid bacteria... How can I perform confirmatory test for it? Meaning how can I check for clear zone of growth inhibition in a petriplate. Thanks

02 March 2016 5,494 11 View

Any suggestions on BMM isolation using M-CSF?

If anyone has a standardized protocol for Bone marrow derived macrophages (BMM) isolation using M-CSF (NOT L929); could you share it with me please?

01 February 2015 6,715 13 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

No title

03 March 2021 5,360 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View