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Questions related from Sreeram Udayan
I am interested in scraping of 100s of colonies from agar plates, and extracting DNA from them directly for subsequent steps of 16s analysis. I want to avoid growing individual colonies due to...
01 January 2017 5,973 18 View
Suppose you want to do adoptive transfer of OT-II cells for checking T cell expansion of a specific population. What difference does it make if one uses cells from OT-II mice vs OT-II Ly5.1+ mice?
11 November 2016 3,344 3 View
Not all antibodies can be used for knockout confirmation since some WB antibodies can bind to both WT and knockout equally (since the antibody recognizes nonmutated regions from the knockout)....
08 August 2016 1,060 4 View
I was coculturing immortalized bone-marrow derived macropahges with Bifidobacteria. Any heads up on how to lyse these cells for plating the lysates to check bacterial persistence within these...
04 April 2016 7,638 2 View
I have a bacteriocin producing lactic acid bacteria... How can I perform confirmatory test for it? Meaning how can I check for clear zone of growth inhibition in a petriplate. Thanks
03 March 2016 5,552 11 View
If anyone has a standardized protocol for Bone marrow derived macrophages (BMM) isolation using M-CSF (NOT L929); could you share it with me please?
02 February 2015 6,807 13 View
