Hello,
I am working on a project about the involvement of oxidative stress in obesity. Does anyone have experience with adiponectin and/or leptin measurement using microplate readers (FluoStar Omega in particular)?
Sorry I just bumped into your question. We have a long experience doing both of these assays in our lab, measuring them in plasma and reading the plates (384-well) with the FluoStar Omega reader. What is it that you need to know? The type of sample, the assay to use or the parametres to set in the reader?
Dear Dr C M Martín Ruiz , thank you for your response, we greatly appreciate it. We want to know if we can use peripheral blood samples, what assay to use, preferably where to purchase reagents from and what protocol to use, as well as what parameters to set in the reader. We would also be willing to collaborate on some research papers if you are interested. We want to measure adiponectin and leptin levels in obese subjects and see what relationship they share with oxidative stress markers.
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