Has anyone ever used activated charcoal to remove pigments during DNA extraction? In which form should it be added? Should it be sterilized? Is efficiency dependent on temperature, pH and time? Could anyone recommend references?
i personally feel activated charcoal .....is not a good option to go with DNA extraction ... you might end up loosing a large amount of DNA ... because charcoal can adsorb DNA efficiently .......
what are the prpolems of pigments presence with the extracted DNA????????
i didn't know that the charcoal captures DNA. I believed that it just captures molecules without electric charge, since it binds substances just covalently, and DNA has a quite negative charge....but thank you for your comment..may be i should look for other reagents for pigment removing..
Thank you for your comments, but i don't understand completely..centrifugation at how many g? in which solvent are them insoluble? in TE? silica spin column sounds good for this problem.
Hi Antonio,
Also interested in charcoal to remove pigments from plant DNA extracts. Do you have an update on your now old posts. Could you use it and if yes which reference did you buy?
Thanks in advance
Stephane
I attempted this with Aedes aegypti whole body DNA and RNA extraction preps. I would lyse the tissue with a small quantity of charcoal and that seemed to remove the pigment. Unfortunately I do not have my notes with me when I did this - so I don't have a specific protocol. I ran RACE rxns and qPCR efficiently afterwards - so it works.
I found centrifugation and spin columns inefficient at removing pigment.
Thanks a lot Richard for your answer. I'll consider it in a future experiment.
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