Admitting to have done some staining applications with acid fuchsin(e)

(CI: 42685), formerly (in my active work-life):

Dear @James Oakes, at least a short search in some sources available either in Histo-books, or in www (especially SDB's /TDS's / >specifications< from main suppliers (like SIGMA-Chemicals or others) will reveal that Acid fuchsine Ca-salt is a 'protocolled' candidate (also 'certified by the Biological Stain Commission', cf.: https://biologicalstaincommission.org/protocols/:

(Copy and Paste:)

ACID FUCHSINE C.I. 42685

Properties

UV-VIS range: 542-546

Min. Dye Content: 60%

Molecular Formula: C20H17N3Na2O9S3 or C20H17CaN3O9S3

Formula Weight: 585.55 or 579.65

Appearance: Green Crystaline Powder

Dye Class: Aminotriarylmethane

Biological Applications: Widely used plasma stain, which also has many other uses including staining connective tissue, mitochondria, and unaborted pollen grains.

Commercial Applications: Textile Dye, dyeing of crepe paper, soap, and photographic film.) (-End of citation-).

Sigma's product page informs:

"certified by the Biological Stain Commission, Dye content ≥60 %

Synonym(e): Fuchsin S Calciumsalz, Rubin S

Lineare Formel: C20H17N3O9S3Ca MG: 579.64

Solubility: CLEAR RED TO VIOLET SOLUTION AT 1 MG PLUS 1ML OF WATER

Certified for use in Van Gieson connective tissue stain, Mallory′s connective tissue stain and Altmann′s mitochondrial stain."

ThermoScientific (Fisher):

Thermo Scientific™ Saures Fuchsin-Natriumsalz

Summenformel: C20H19N3Na2O9S3

Molekulargewicht (g/mol)

587.544

Acid Fuchsin sodium salt is used as a pH indicator. It is one of the dyes used in Masson’s Trichome Stain where it is used to distinguish muscle from collagen. Solubility in water: (1 mg/ml)

Synonyms: acid fuchsin, acidal fuchsine, acid fuchsine, acidal magenta, fuchsine acid, fuchsin acid, p-fuchsine acid, acid fuchsine n, acid fuchsine o, acid fuchsine s

https://www.scbt.com/de/p/acid-fuchsin-calcium-salt-123334-10-1

>>Acid fuchsin calcium salt (CAS 123334-10-1), a stain certified for use in Altmann′s mitochondrial stain, from Santa Cruz….Alternative names/synonyms: ==>Acid Magenta; Acid Rubin

Acid fuchsin calcium salt is certified to use in Altmann′s mitochondrial stain, Mallory′s connective tissue stain and Van Gieson connective tissue stain.[MW: 579.63 / MF: C20H17N3O9S3 Ca

Literaturhinweise / References:

1. Penney, D.P., et al. 2002. Biotech. Histochem. 77: 237-275. PMID: 12564600

Hopefully you will/are able top cope with the handling of the needed picric acid (;-))...

[Van Gieson Stain - VGS:

Picric Acid , saturated aqueous solution.....50 ml

1.0% acid fuchsin, aqueous solution ........... 9 ml

A. dest. / distilled water ...............................50 ml

Learning about the properties / description of Acid fuchsin(e), as described in CONN's Biological Stains(A Handbook of Dyes, Stains, and Fluorochromes for Use in Biology and Medicine), 10th Ed.; ed. by R.W HOROBIN and J.A. KIERNAN (2002 Published for the Biological Stain Commission by BIOS, Oxford UK) on further request.

From Salzburg, Austria - My best wishes...and good luck, WHM.