I have mice brain alices with FG staining without the use of anti-FG antibody. I have some very brightly stained neurons while quite a few somewhat faded cells in the background.
Should i count only the brightly stained neurons only or also the ones which show any weak signal. I worry I will have lots of false positives then.
Ute Neubacher
You should check this by the use of higher magnification because the FG fluorescence has a crystalline structure which is even in not so intensive stained cell well distiguishable.
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