Hi Arslan,

• Debris after passage is often due to stress from over-dissociation — shorten enzymatic steps and handle cells gently.
• Keep ROCK inhibitor for longer than 24 h if viability is low; abrupt withdrawal can trigger massive cell death.
• Ensure your PLO + laminin coating is fresh and rinsed well; coating variability strongly impacts attachment.
• For more consistent adhesion and less debris, DendroTEK dPGA | Protease-Resistant Culture Matrix offers a stable alternative to conventional coatings like PLO. Essentially use dPGA as your first coating and then overlay with laminin.

Best of luck,

Jason Hamlin

Source: I work at DendroTEK Biosciences. www.dendrotek.ca