I have faced a problem with culturing Ac16 cardiomyocytes cell line.
Day 0 I plated 200,000 cells/2ml (well) in 6 wells plate in FGM.
Day 3 I put my cells on Low serum medium.
When I changed the medium for the cells, the cells detached from the plate, and I can see them floating in the medium. Why that happened, and what would be the best way to prevent that?
Dear Shrouq!
I think it happened because you were using a low serum medium.
You look at following article, please:
Article Dynamic reorganization of the AC16 cardiomyocyte transcripto...
Methods Cell culture and treatments AC16 human adult ventricular cardiomyocyte cells [52] were purchased from the American Type Cell Culture (ATCC). The cells were maintained in DMEM F-12 supplemented with 12.5% fetal bovine serum.
Alexandr Chernov
Hi Alexander,
thank you very much for the paper you have sent, it is really informative.
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