Does anyone know the abs/em of qubit's broad range dsdna assay? I have a lot of leftover reagents, and no qubit, but I do have access to a plate reader.
Dear Sir. Concerning your issue about the absorbance / emission of qubit BR DNA assay . Based on a simple mix-and-read format, Invitrogen Qubit assays can be read in the Qubit Fluorometer following as little as two minutes' incubation. The dye in each Qubit Assay Kit emits fluorescence only when specifically bound to the target molecule, which minimizes the effects of contaminants—including degraded DNA or RNA—on the result. The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent 1:200, load 200 μL into the wells of a microplate, add 1–20 μL sample volumes, mix, then read the fluorescence. The assay is highly selective for double-stranded DNA over RNA, and in the range of 0.2–100 ng, the fluorescence signal is linear with DNA (Figure 1). The assay is performed at room temperature, and the signal is stable for 3 hours. Common contaminants, such as salts, solvents, detergents, or protein are well tolerated in the assay I think the following below links may help you in your analysis:
http://genecore3.genecore.embl.de/genecore3/downloads/Solexa/Quant-iT-dsDNA-protocol.pdf
http://www.thermofisher.com/sa/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-assays.html
http://andersenlab.org/Protocols/96-wellDNAQuantification_Synergy4_Qubit_v2.pdf
Thanks
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