Hi folks,
I've heard of issues with AAV double transduction, i.e. where one wants to express two independent genes-of-interest in one cell (either in vivo or in vitro). For example, an 'AAV-Cre-mCherry' virus with an 'AAV-Calcium indicator' or 'AAV-GFP' and cannot find any or only few cells that express both vectors.
(This is NOT about double transduction to have expression of a single larger construct, which would be split into two dependent AAV vectors to circumvent the AAV size limitation.)
I would like to hear from you here to get a better understanding of what might cause these issues, specifically what vectors you used (vector map, Addgene link, etc).
Thanks for your help!
Cheers
I am interested in this, did you got some comments from elsewhere?
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