Why is my primer amplifying multiple products despite specificity, yet resolved with SNaPshot?

I have several COMT SNPs which I designed primers for using BatchPrimer3. I did all my checks and did a BLAST for all primers to ensure it binds to the correct region. All primers have worked...

14 September 2020 4,143 4 View

ABC elite kit - should I agitate while incubating tissue with ABC?

I inherited a staining protocol from another lab. It specifically says not to swirl the ABC mixture, but instead to let it sit for 30 minutes before adding the tissue. It does not specify if the...

26 January 2020 5,868 2 View

Plasmid Problems: Is it possible to use RE to cut a section out of a plasmid & immediately amplify it?

I have a 1.6kb insert that has been cloned into a plasmid. I would like to cut a section out of this plasmid that is flanked by restriction sites for ECOR1. Would it be possible to digest the...

11 February 2019 2,848 7 View

Convert a 3D direction vector to yaw and pitch?

I am trying to convert 3D direction vectors (see example) to yaw and pitch (apparently you can't get roll from the vectors). My ultimate goal is to determine the Euler angles from my answers....

09 September 2018 1,472 1 View

Using the gromacs md run -rerun command, is it possible to generate bonded terms?

Hi, I ran some MD simulations in GROMACS and am trying to get a sense of the changes in potential energy of the active site between conditions (with and without inhibitor present). I have...

25 April 2018 7,688 5 View

Running MD simulations on small molecules and generating parameters ?

I am generating small molecule parameters using PRODRG and ABT. Why exactly does PRODRG take less time then ABT? I want to know this because I am trying to decide if it is worth it to wait for ABT...

03 April 2018 5,705 2 View

Can you detect secreted ATP by luminescence if your supernatans contains FBS?

26 April 2017 3,760 5 View

Is there a method to score therapist interventions or attitudes in CBT groups?

I'm looking for a scoring system that differentiates therapists interventions in CBT groups. I want to know what kind of attitude, interpersonal style works in treating batterers. The specific...

08 September 2016 6,613 1 View

IFN-g staining: Does someone know a good IFNg antibody to stain human tissue?

09 February 2016 4,411 2 View

Plagiarism-somebody stolen part of my PhD thesis, what should I do?

If somebody copies a huge part from your own article or thesis, and publish it on a journal as if it is his own work, what should you do? Does anybody experience this?

01 February 2016 844 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View