Dear Sir. Concerning your issue about a method to extract mitoTEMPO from plasma and tissue samples. I think the following below links may help you in your analysis:

https://www.hindawi.com/journals/omcl/2017/6412682/

http://jap.physiology.org/content/jap/120/7/733.full.pdf

http://www.sciencedirect.com/science/article/pii/S0891584917300126

Thanks

Dear Nisha;

I think the following below paper may help you in your analysis.

"Imaging of Superoxide Generation in the Dopaminergic Area of the Brain in Parkinson’s Disease, Using Mito-TEMPO"

Good luck

Thank you both Isam and Feras for the papers. However, I couldn't find any information on extraction and analysis of mito-TEMPO by LCMS/MS in them.